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For TUDCA treatment, a dose of 5 mg/kg of TUDCA was administered intraperitoneally 1 h before LPS treatment. Vehicle control mice received the same amount of sterile saline. At specific time points following LPS administration, the syringe assembly (MSA-250-M, Penn Century, USA) accordingtothepreviousstudy[15]andEApretreatmentat nonacupoint for 45 minutes; LPS group, where the rats received intratracheal instillation of lipopolysaccharide (LPS; L2630, Sigma MO, USA, 0.4mg/kg, 100ul) by a MicroSprayer syringe assembly and EA pretreatment at View and Download Ahuja SSA-250 M operation manual online. SSA-250 M amplifier pdf manual download. Also for: Br-250m. Mice were kept on a low fluorescent diet (AIN-76A; Brogaarden Korn & Foder ApS, Denmark) for at least 10 days before treatment.
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A MicroSprayer (syringe assembly, MSA-250-m, the Penn Century Inc., PA, USA) was used to deliver all materials to the lungs via i.t. Mice (20–30 g) were exposed to 0.25 U of PPE (on days 1, 7, and 14) and 7.0 μg of LPS (on days 4, 11, and 18) for three consecutive weeks. syringe assembly (MSA-250-M, Penn Century, USA) accordingtothepreviousstudy[15]andEApretreatmentat nonacupoint for 45 minutes; LPS group, where the rats received intratracheal instillation of lipopolysaccharide (LPS; L2630, Sigma MO, USA, 0.4mg/kg, 100ul) by a MicroSprayer syringe assembly and EA pretreatment at MIC2H5, MILFXX2004718, MSA-250-M, MWA3848, N3050126, NC0504811, NC0915729, NU-425-200, P100N, P4675-11X. Get a quote and buy Laboratory Equipment and Supplies.
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ALI control mice (Veh) received the same amount of sterile saline. For TUDCA treatment, a dose of 5 mg/kg of TUDCA was administered intraperitoneally 1 h before LPS treatment. Vehicle control mice received the same amount of sterile saline. At specific time points following LPS administration, the Briefly, following anesthetization with pentobarbital, 50 µg of LPS in a volume of 25 µL of saline was delivered to the mice intratracheally using a MicroSprayer syringe assembly (MSA-250-M, Penn Century, USA). ALI control mice (Veh) received the same amount of sterile saline. Aerosolizer – Model MSA-250-M (Penn-Century. Inc.) with a volume of 50 μl/mouse by placing the aerosolizer tip in the trachea immediately proximal to the carina and rapidly depressing the plunger.
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